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Polyherbal therapy is said to be a current pharmacological principle having the advantage of producing maximum therapeutic efficacy with minimum side effects. ). Piper betle Linn. (Piperaceae) is a perennial dioecious creeper, cultivated in India for its leaves, used for chewing. The leaf is carminative, aphrodisiac, tonic, laxative and improves appetite. Leaves contained caryophyllene, cadinene, γ-lactone, allyl catechol, p-cymene and eugenol methyl ether in varying amounts. Cynodon dactylon (L.) Pers. (Fam: Gramineae) is commonly known as “Doob” in India (Arugampul: Tamil). It is a weed and possesses varied medicinal properties. catalase, glutathione, Peroxidase. Certain vitamins, minerals, herb sand other nutritional substances also perform as antioxidants. The antimicrobial properties of several naturally occurring compounds have been known for decades. The compounds are eluted by using Chloroform, Toluene and Ethyl acetate and chloroform and ethyl acetate for Cynodon dactylon in the ratio of (0.6) , (0.76). The isolated compound from Cynodon dactylon was brow color amorphous powder and characterized through IR, NMR, and mass spectral data of interpretation and the compound may be 1-methyl ethyl propanoate and 5-phenyl-3,4-alkynyl-2-pentanone. The antibacterial assay was performed by Cup-plate method. Thus, such bore contain 500 μg of Cynodon dactylon (L) pers, and Piper betle of individual and for combined sample 250 μg of each isolate. To compare the activity with standard antibiotics, Ciprofloxacin ( 30 μg) was used. In summary, this study had shown that despite the high values of Inhibition by combining the two isolates, Cynodon Dactylon and Piper Betle could be used for treatment of the skin infection (topical).
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