Expression pattern of pro- and anti-apoptotic proteins in patients with squamous cell carcinoma of the uterine cervix

  • Krishnan Baskaran Division of Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India
  • Ramanathan Satish Division of Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India
  • Viswan Santhosh Division of Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India
  • Radhika Govindaraju Division of Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India
  • Shanmugam Manoharan Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India
  • Subramanian Sethupathy S Division of Biochemistry, Rajah Muthiah Medical College, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India

Abstract

Apoptosis is a complex pathway, which plays a central role in the physiological processes. Aberrant regulation of apoptosis or defective expression of apoptotic- related proteins might result in tumourigenesis. The present study investigated the expression pattern of Bcl-2 and Bax by immunohistochemistry and the activities of caspase-3 and -9 by ELISA reader in patients with squamous cell carcinoma (SCC) of the uterine cervix. Bcl-2 expression was found in 65% of the tumour cells while Bax expression was found in 45% of the tumour cells. The activity of caspase-3 was significantly reduced (p<0.001) while the activity of caspase-9 was unaltered in patients with SCC (p>0.05) as compared to normal subjects. The results thus suggest defective expression of pro- and anti- apoptotic proteins in patients with SCC of the uterine cervix, which could play a role in the abnormal cell proliferation of the uterine cervix.

Keywords: Apoptosis, Bcl-2, Bax, Caspase, Cervical cancer

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Published
2010-10-22
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Krishnan Baskaran, Ramanathan Satish, Viswan Santhosh, Radhika Govindaraju, Shanmugam Manoharan, & Subramanian Sethupathy S. (2010). Expression pattern of pro- and anti-apoptotic proteins in patients with squamous cell carcinoma of the uterine cervix. International Journal of Research in Pharmaceutical Sciences, 1(4), 546-550. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/965
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