Simultaneous estimation of colorants Sunset Yellow and Tartrazine in food products by RP-HPLC

  • Krishna Veni N Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India
  • Meyyanathan S. N Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India
  • Narendra Babu B Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India
  • Abhishek Kumar Sharma Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India
  • Arun Srikanth B Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India
  • Bombhroliya Satyam A Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India
  • Suresh B Department of Pharmaceutical Analysis, J. S. S. College of Pharmacy (Off Campus - JSS University, Mysore), Ootacamund, Tamilnadu, India

Abstract

An accurate, rapid and sensitive RP-HPLC method was developed and validated to simultaneously estimate sunset yellow and tartrazine in food products. Analysis was performed using waters 1515 isocratic HPLC system (pump); A Waters 2487 dual wavelength detector was used for analysis with a Waters Breeze version 3.3 data station for data collec-tion and processing. Separation was achieved using a Phenomenex C18 column, (250 × 4.6mm, 5µ), A mixture of 50 mM potassium dihydrogen orthophosphate buffer (adjusted to pH 7.5): acetonitrile (80: 20v/v) was pumped at 0.7 mL/min for separation. The eluents were monitored at 244 nm. A solution containing 0.5gm of both Sunset yellow and Tartrazine were prepared from a commercially available food product and then used for evaluation. 15µg/ml solution of riboflavin was used as internal standard. The developed method was validated as per ICH guidelines. The separation with the above said conditions gave a sharp and symmetric peak. Linearity for Tartrazine and Sunset yellow were observed in the concentration range of 0.5 – 3.0 µg/mL for both with a correlation of 0.999. The recoveries were found to be 85.5 and 83.3 % respectively. In conclusion a simple, sensitive and rapid RP HPLC method was developed for simultaneous estimation of Tartrazine and Sunset yellow in food products.

Keywords: Sunset yellow, Tartrazine, RP-HPLC, Validation, Food analysis

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Published
2011-10-20
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How to Cite
Krishna Veni N, Meyyanathan S. N, Narendra Babu B, Abhishek Kumar Sharma, Arun Srikanth B, Bombhroliya Satyam A, & Suresh B. (2011). Simultaneous estimation of colorants Sunset Yellow and Tartrazine in food products by RP-HPLC. International Journal of Research in Pharmaceutical Sciences, 2(4), 545-549. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/941
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Original Articles
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