Method development and validation of hydrochlorothiazide in tablet dosage form by UV spectroscopy

  • Alagar Raja M Dept. of Pharmaceutical Analysis, Karpagam University, Coimbatore, Tamil Nadu, India
  • Selvakumar D AVN Ayurvedic Research Centre, Madurai, Tamil Nadu, India

Abstract

A simple, reproducible and efficient spectroscopic method development and validation of hydrochlorothiazide in tablet dosage form. The drug was ermined by using methanol as a solvent for this study, which is determined by spectrophotometrically at 224-nm.the percentage recovery study of the drug for the proposed method range from 99-100%w/v indicating no interferences of the tablet excipients, by using methanol as a solvent. Linearity was obtained in the concentration range 10-50 µg/ml for the hydrochlorothiazide with correlation coefficient of 0.9916. The result analysis was validated statistically and recovery studies confirmed the accuracy and precision of the proposed method.

Keywords: Hydrochlorothiazide, Methanol, UV spectrophotometer

Downloads

Download data is not yet available.
Published
2010-07-25
Citations
How to Cite
Alagar Raja M, & Selvakumar D. (2010). Method development and validation of hydrochlorothiazide in tablet dosage form by UV spectroscopy. International Journal of Research in Pharmaceutical Sciences, 1(3), 369-371. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/870
Section
Original Articles
Article Level Metrix
Abstract Views: 7
PDF Downloaded: 3