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A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Abacavir in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of 0.1%orthophosphoric acid: acetonitrile: methanol 10:25:65 (V/V). The UV detection wavelength was 259nm and 20µl sample was injected. The retention time for Abacavir was 7.005 min. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Abacavir in tablet dosage form and in serum.
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