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A simple, selective, rapid, and precise RP-HPLC-PDA method has been developed for the simultaneous estimation of Lornoxicam (LOR) and Thiocolchicoside (THIO) in pharmaceutical dosage form by reverse phase liquid chromatography using Waters Symmetry C18 (250 mm × 4.6 mm, 5.0 µ) column. The mobile phase consisting of methanol: THF: acetate buffer (60: 10: 30 v/v); pH adjusted to 5.5 with glacial acetic acid at a flow rate of 0.75 mL min-1 and column was maintained at 50 0C with detection at 382 nm. The retention time of Thiocolchicoside and Lornoxicam was 3.36 and 4.08 minutes, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness, limit of detection and limit of quantification. Linearity of Lornoxicam and Thiocolchicoside were in the range of 0.2 to 80 μg mL-1 and 0.1 to 40 μg mL-1, respectively and its percentage recovery were found to be 100.37 % and 100.51 %, respectively. The proposed method is suitable for simultaneous determination of Lornoxicam and Thiocolchicoside in pharmaceutical dosage form. Method was successfully applied for dissolution study of tablet formulation.


Lornoxicam Thiocolchicoside RP-HPLC-PDA Method validation Column Liquid Chromatography Dissolution

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How to Cite
Madhusmita Sahoo, Pratima Syal, Snehal Ingale, Kunal Ingale, Santosh Sindhe, Monali Sali, V.P. Choudhari, & B.S. Kuchekar. (2011). Development and Validation of a RP-HPLC-PDA method for Simultaneous Determination of Lornoxicam and Thiocolchicoside in Pharmaceutical dosage form and it’s Application for Dissolution study. International Journal of Research in Pharmaceutical Sciences, 2(1), 1-7. Retrieved from