Development and Validation of a HPLC Method for Determination and Quantification of α-mangostin in Bark Extract of Garcinia cowa Roxb
Kandis, Garcinia cowa Roxb, traditionally has been used for many purposes. Many parts of G. cowa have been used in traditional folk medicine as antipyretic and anti-inflammatory. α-mangostin represents the majority of the clinical benefits of this herbal medicine. It is reasonable and logical to determine the concentration of α-mangostin as a chemical marker for the quality control of G. cowa and its products. The aim of this study was to set up a validated and stability-indicated isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method for quality control and quantity determination of α-mangostin from ethanol extract of G. cowa. The assay was fully validated and shown to be linear (r2 = 0.999), sensitive (LOD = 0.04 μg/ml and LOQ = 0.16 μg/ml) and precise (intra-day variation ≤ 1.6 %, inter-day variation ≤ 4.3%). Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 86.67%. Total analysis was ~ 15 min. The present method should be useful for analytical research and for routine quality control analysis of α-mangostin in ethanol extract of G. cowa.
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