Expression of cholera toxin B antigen (CT-B) epitope in transgenic edible banana plant (Robusta sp.)

  • Saravanan R JNTUK, Kakinada and Faculty, QISCP, Vengamukalapalem, Ongole-533003, Andhra Pradesh, India
  • Dhachinamoorthi D QISCP, Vengamukalapalem, Ongole-523272 Andhra Pradesh, India
  • Sudhakar Y Govt Polytechnique College, Tirupathi-511750, Andhra Pradesh, India

Abstract

Vibrio cholerae is a key purpose behind Cholera illnesses, and how to annihilate Cholera has been examined for a very long while. Oral antibodies delivered by transgenic plants would change the customary methods for generation and immunization of Cholera antibodies and lessen the inoculation cost essentially. In this examination interestingly we utilized the transgenic Banana (Robusta sp) framework to express Vibrio cholerae epitope immunization CTB, a recombinant peptide which could secure Cholera contamination after oral inoculation by expanding the particular antibodies. CTB was changed into Banana plant interceded by Agrobacterium tumifacience LBA4401, and transgenic shoots have been confirmed by PCR investigation, Western blot and ELISA examination demonstrated that CTB quality could transcript and create the objective peptide in the Leaf and Fruits. Transgenic Banana plant and their parts like leaf and Fruit product inferred oral antibody procedure could be conceivably utilized as an option system to battle for the Cholera contamination, particularly for the general population in remote and non-developed country.

Keywords: Cholera, CT-B, p CAMBIA, Edible Vaccine, ELISA, Transgenic Banana, Robusta sp.

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Published
2017-07-10
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How to Cite
Saravanan R, Dhachinamoorthi D, & Sudhakar Y. (2017). Expression of cholera toxin B antigen (CT-B) epitope in transgenic edible banana plant (Robusta sp.). International Journal of Research in Pharmaceutical Sciences, 8(3), 442-450. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/635
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Original Articles
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