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Nyctanthes arbor-tristis linn (N. arbor-tristis) leaves was extensively used in Ayurveda medicine system for the healing of numerous aliments and also found to contain pharmacological and biological activities. The study was aimed to compare the antioxidant potential present in the N. arbor-tristis leaves. The fresh leaves of selected plant N. arbor-tristis were subjected to preliminary phytochemical qualitative analysis by specific methods. These phytochemical qualitative analysis indicated the existence of Carbohydrates, Terpenoids, Flavonoids, Glycosides, Tannins, Saponins, and absence of Alkaloids and Phlobatannins. Along with these the level of enzymatic antioxidants for example catalase, peroxidase and glutathione-s-transferase, and non-enzymatic antioxidants for example polyphenol, ascorbic acid and total carotenoids are also estimated. The outcome demonstrated a significant level of P<0.001 in catalase, polyphenol and total carotenoids when compared to peroxidase, glutathione-s-transferase and ascorbic acid with a significance of P<0.05.
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