Free radicals scavenging and antiproliferative activity of ethanolic and methanolic extracts of Carica papaya leaves in PA-1 cell line

  • Priyadurairaj Dr.M.G.R. Educational and Research Institute University, Chennai-95. Tamil Nadu, India
  • Palagati Rohith Kumar Reddy Dr.M.G.R. Educational and Research Institute University, Chennai-95. Tamil Nadu, India
  • Palaniyandi Thiruvanavukkarasu Dr.M.G.R. Educational and Research Institute University, Chennai-95. Tamil Nadu, India
  • Rajeswary Hari Dr.M.G.R. Educational and Research Institute University, Chennai-95. Tamil Nadu, India

Abstract

A comparative antioxidant and anticancer potential of methanolic and ethanolic extracts of leaves of Carica papaya were investigated. Both the plant extracts were prepared separately, and their anti-oxidant scavenging potential was investigated for the scavenging of superoxide, nitric oxide and hydrogen peroxide radicals using standard protocols. The anticancer property of these plant extracts was evaluated in PA-1 cell lines using tryptan blue assay. The total phenols and flavonoids contents responsible for the above said activities were estimated quantitatively. Among the two extracts, the ethanolic extract scavenged the free radicals to a greater extent when compared to the methanolic extract. Similarly, in the tryptan blue exclusion assay, the percentage of cell death was more for ethanolic extract. At high concentrations such as 50, 75 and 100 μg concentrations, the ethanolic extract showed higher cell death which was comparable to the positive drug doxrubicidin used in the present study. Both the extracts possessed considerable quantities of phenols and flavonoids. It may be concluded that the presence phytochemicals along with the phenols and flavonoids may be the reason for the antioxidant and anticancer activity observed in the present study.

Keywords: Chemotherapy, Deoxyribose sugar, ROS, RNS, Antitumour activity

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Published
2019-01-05
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Priyadurairaj, Palagati Rohith Kumar Reddy, Palaniyandi Thiruvanavukkarasu, & Rajeswary Hari. (2019). Free radicals scavenging and antiproliferative activity of ethanolic and methanolic extracts of Carica papaya leaves in PA-1 cell line . International Journal of Research in Pharmaceutical Sciences, 10(1), 339-345. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/57
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Original Articles
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