Spectrophotometric determination of neomycin sulphate in tablets form via reaction with ninhydrin reagent

  • Khalaf F Alsamarrai Department of Chemistry, College of Education, University of Samarra, Samarra, Iraq
  • Menaa Abdulsalam Al-Abbasi Department of Chemistry, College of Applied Science, University of Samarra, Samarra, Iraq
  • Eman Thiab Alsamarrai Department of Chemistry, College of Education, University of Samarra, Samarra, Iraq

Abstract

A new, sensitive, simple and cheap spectrophotometric method for the determination of Neomycin Sulphate (NEO) in pharmaceutical forms has been developed. The method is based on the reaction between NEO and NIN in basic medium. The maximum absorbance was at 574 nm. The conditions affecting the reaction were optimized. Under the optimal conditions, the calibration curve was linear over the range of 0.0002-0.0011 mol/L. The limit of detection and limit of quantification were 5.423×10-6 mol/L, and 1.643×10-5 mol/L, RSD% of seven replicate was 0.8217- 0.8321% and Rec% was between 99.2168-100.8857%. The proposed method was successfully applied to the determination of NEO tablets form.

Keywords: Spectrophotometric, Neomycin sulphate, Ninhydrin reagent, Neomycin tablets

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Published
2019-04-15
Citations
How to Cite
Khalaf F Alsamarrai, Menaa Abdulsalam Al-Abbasi, & Eman Thiab Alsamarrai. (2019). Spectrophotometric determination of neomycin sulphate in tablets form via reaction with ninhydrin reagent . International Journal of Research in Pharmaceutical Sciences, 10(2), 1392-1396. https://doi.org/10.26452/ijrps.v10i2.548
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Original Articles
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