Main Article Content

Abstract

The resistance of methicillin-resistant staphylococcus aureus (MRSA) to antimicrobials drugs is due to expression of the mecA gene. Current study was conducted on 33 MRSA clinical samples (Cefotaxime and Oxacillin positive). All MRSA isolates were examined using Antibiogram, Minimum Inhibitory concentration (MIC) and PCR to clarify the expression of SCCmec genes and to detect the differences on repeats of coagulase gene (Coa). Results showed that all isolates were 100% resistant against Amoxycillin-clavulanate, Ampicillin and Cefotaxime, 45.5% were resistant against Ciprofloxacin and erythromycin. mecA gene is expressed in all examined isolates (100%). The expression of SCCmec genes showed that 11.11% expressed type I, 45.45% contained type II, 45.45% contained type III, 63.63% were type IV and 5.55% were typeV. All examined isolates harbored and expressed coagulase gene repeats. Coagulase repeats were 27.27% with 5 repeats (81pb), and 72.72% with 4 repeats. In conclusion, the virulence of MRSA strains is increased and gave different antibiogram activities from different global regions and the repeats of Coa gene give no detectable differences among MRSA strains.

Keywords

MRSA Chromosome Cassette patterns Coagulase repeats PCR

Article Details

How to Cite
Salama Mostafa Abdel-Hafez, Essam Hassan Mohamed, Youssef Saeed Alghamdi, Mohamed Mohamed Soliman, Saad Hommod Alotaibi, Adel Alkhedaide, Magdy Yassin Hassan, & Hamada Hassan Amer. (2021). Assessment of Chromosome Cassette Pattern of MRSA in Correlation to Coagulase Gene. International Journal of Research in Pharmaceutical Sciences, 12(3), 2207-2214. https://doi.org/10.26452/ijrps.v12i3.4835