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During the present study effect of Vitamin E and Tinospora cordifolia on BPA induced goat (Capra hircus) testicular tissue was evaluated. Testicular tissue was exposed to varied concentrations of BPA, Vitamin E and Tinospora cordifolia in three experimental groups along with their specific control for 4 and 8hrs. In group I, the cultured tissue was exposed to three doses of Bisphenol-A viz. 0.01, 1.0 and 100 nM/ml. In group II, Vitamin E (0.1µM/ml) was supplemented along with BPA (0.01, 1.0, 100 nM/ml) and in group III Tinospora cordifolia extract (250 µg/ml) was added with BPA (0.01, 1.0, 100 nM/ml) in the culture media. With the increase in dose level as well as the exposure duration, the frequency of apoptotic cells increased significantly (p< 0.05), as revealed by acridine orange and methylene blue staining. BPA exposure also induced a decline in the antioxidant enzyme activity such as catalase, glutathione peroxidase and reduced glutathione. Vitamin E and Tinospora cordifolia resulted in a decrease in the percentage of apoptotic cells and an increase in levels of catalase, glutathione peroxidase and reduced glutathione activity was observed. Hence, it is concluded that BPA induced more damage at its higher concentration and the antioxidant Vitamin E and Tinospora cordifolia ameliorate the oxidative stress-induced changes, thereby suggesting the potential of Vitamin E and Tinospora cordifolia in counteracting the cellular damage induced by agents like BPA.
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