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From the ancient days itself the ayurvedic medicine has special importance. This ayurvedic system of medicine originated in India. In India nearly 70% of people use ayurvedic system of medicine along the western system of medicine. The Ayurveda mainly come under three classes are vata, pitta and the kapha. Chemically identified constituents of an herbal medicine that are of concern for quality control purposes are known as markers. Some of the markers are widely used for anti-inflammatory agents are Boswellia, sunthi, gokshuru, guduchi, guggulu this marker possess anti-inflammatory action that reduces pain, swelling of joints, Arthritis, joint complaints, Muscular injuries etc. The chromatographical / spectroscopical analytical development can be done for markers. The analytical development possess the way to the analytical information about the markers. The technique such as HPLC, HPTLC, LC-MS, UPLC-PDA are properly validated by ICH guidelines using validation parameters such as accuracy, recovery, precision, LOD, LOQ, Robustness etc. For many markers there is lack of development of Hyphenated techniques. By increasing the analytical techniques, research is prone to be more effective and successful to promote quality, safety and efficacy.
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