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Abstract

Doxorubicin is a chemotherapeutic agent with a genotoxic effect on normal cells at its therapeutic dose itself. D-Pinitol is an abundantly available carbohydrate in Soybean plants and has been proven for antioxidant and anti-inflammatory activities. Our investigation was examined by in vitro comet assay to explore the genoprotective effect of D-Pinitol in normal cells against Doxorubicin-induced genotoxicity in Vero cell lines. In vitro comet assay treatment groups were: Vero Cell lines with culture medium (control group), Doxorubicin (0.15μg/ml), D-Pinitol (0.05×103 mM, 0.125×103 mM, and 0.25×103 mM) alone, and pretreatment with D-Pinitol (0.05×103 mM, 0.125×103 mM, and 0.25×103 mM) before Doxorubicin (0.15μg/ml) treatment. When compared to the control group, D-Pinitol alone treated groups showed no significant changes in the percentage of DNA damage. For the evaluation of the genoprotective effect of D-Pinitol, the % DNA damage in the D-Pinitol, and Doxorubicin simultaneously treated groups were compared to the Doxorubicin alone treated group. The results showed that Doxorubicin-induced genotoxic effect in Vero cell lines was significantly reduced by D-Pinitol in a dose-dependent manner by reducing DNA damage. Our findings confirmed that D-Pinitol had no genotoxic effect and it showed a genoprotective effect against Doxorubicin-induced genotoxicity. 

Keywords

Genotoxicity Doxorubicin D-Pinitol In vitro Comet assay

Article Details

How to Cite
Murugesan Sudha, & Thangarasu Vetrichelvan. (2021). Genoprotective effect of D-Pinitol isolated from aerial parts of Soybean plants against Doxorubicin-induced genotoxicity evaluated by in vitro comet assay in Vero cell lines. International Journal of Research in Pharmaceutical Sciences, 12(2), 1379-1384. https://doi.org/10.26452/ijrps.v12i2.4694