Ultrasonic dopplerography for the evaluation of endothelial function in the conduct of pharmacological vascular samples in an experiment

  • Vladislav O. Soldatov Department of Pharmacology, Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
  • Tatyana N. Malorodova Department of Pharmacology, Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
  • Tatyana G. Pokrovskaya Department of Pharmacology, Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
  • Mikhail V. Pokrovskii Department of Pharmacology, Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
  • Tatyana I. Kulchenkova Department of Pharmacology, Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
  • Andrey O. Ksenofontov Department of Pharmacology, Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
  • Olga V. Filippova Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, 2-4 Bolshaya Pi-rogovskaya st., 119991 Moscow, Russia

Abstract

To study the function of the endothelium in conducting vascular pharmacological samples in normal and blocking the synthesis of nitric oxide by ultrasound examination of the velocity of central blood flow in the femoral artery in comparison with the changes in systemic hemodynamics and the linear velocity of microcirculatory flow using laser flowmetry. It was studied the parameters of the blood flow velocity (the maximum systolic and diastolic velocity, average systolic and diastolic velocity, Gosling index of pulsatility and resistance index by the Doppler ultrasound Minimax-Doppler-K), the parameters of central circulation ("Biopac-systems MP-150", AcqKnowledge 4.2, USA), and linear velocity of the microcirculatory flow of the musculus vastus medialis (Biopac MP-150, USA) in intact animals and rats with N-Nitro-L-arginine methyl ester (L-NAME) induced deficiency of nitric oxide. Quantitative evaluation of endothelial dysfunction in the conduct of pharmacological tests with endothelium-dependent (acetylcholine) and endothelium-independent (sodium nitroprusside) vasodilatation is most indicative in calculating the coefficient reflecting the ratio of vascular responses areas in intact rats with L-NAME induced deficiency of nitric oxide. Blood flow parameters (the maximum systolic velocity, the calculated difference between systolic and diastolic velocities) showed a high correlation, both with mean arterial pressure and a linear velocity of the microcirculatory flow. We conclude, that ultrasound Doppler were reflected in the systemic and local vascular response to the administration of vasodilators. It allows an assessment of endothelial function by using the Minimax-Doppler-K device.

Keywords: Minimax Doppler, Blood flow, Endothelial dysfunction, Nitric oxide, Acetylcholine, L-NAME

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Published
2018-07-12
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How to Cite
Vladislav O. Soldatov, Tatyana N. Malorodova, Tatyana G. Pokrovskaya, Mikhail V. Pokrovskii, Tatyana I. Kulchenkova, Andrey O. Ksenofontov, & Olga V. Filippova. (2018). Ultrasonic dopplerography for the evaluation of endothelial function in the conduct of pharmacological vascular samples in an experiment. International Journal of Research in Pharmaceutical Sciences, 9(3). Retrieved from https://pharmascope.org/index.php/ijrps/article/view/303
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