Method development and validation for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine by RP-UPLC in pharmaceuti-cal dosage form

  • Sivakamasundari G Department of Pharmacy, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India
  • Kannappan N Department of Pharmacy, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India

Abstract

A novel, simple, accurate reverse phase ultra-performance liquid chromatographic method for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine in pharmaceutical dosage form was developed and validated. This method was performed on Thermo scientific-Hypersil gold C18, 1.9µm (50mm X 2.1mm) column with Mobile Phase 60 volume of buffer and 40 volume of Acetonitrile. Buffer Solution was mixing 1ml of O-Phosphoric acid in 1000ml of water. The flow rate was 0.3 ml/min and detection were carried out using a PDA detector at 232 nm. The retention times were 2.6, 3.0, & 3.7 min for Chlorthalidone, Telmisartan and, Amlodipine respectively. The method was linear over the concentration range of 8-12μg/ml for Chlorthalidone and 24-36µg/ml for Telmisartan and 3-7µg/ml for Amlodipine with a correlation coefficient of 0.999, 0.998 and 0.997 respectively. The developed method was validated as per ICH guidelines for linearity, accuracy, precision, robustness, specificity, limit of detection, limit of quantitation.

Keywords: Telmisartan, Chlorthalidone, Amlodipine besylate, RP-UPLC, Simultaneous Estima-tion

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Published
2018-07-12
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How to Cite
Sivakamasundari G, & Kannappan N. (2018). Method development and validation for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine by RP-UPLC in pharmaceuti-cal dosage form. International Journal of Research in Pharmaceutical Sciences, 9(3). Retrieved from https://pharmascope.org/index.php/ijrps/article/view/294
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Original Articles
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