Method development and validation for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine by RP-UPLC in pharmaceuti-cal dosage form
A novel, simple, accurate reverse phase ultra-performance liquid chromatographic method for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine in pharmaceutical dosage form was developed and validated. This method was performed on Thermo scientific-Hypersil gold C18, 1.9µm (50mm X 2.1mm) column with Mobile Phase 60 volume of buffer and 40 volume of Acetonitrile. Buffer Solution was mixing 1ml of O-Phosphoric acid in 1000ml of water. The flow rate was 0.3 ml/min and detection were carried out using a PDA detector at 232 nm. The retention times were 2.6, 3.0, & 3.7 min for Chlorthalidone, Telmisartan and, Amlodipine respectively. The method was linear over the concentration range of 8-12μg/ml for Chlorthalidone and 24-36µg/ml for Telmisartan and 3-7µg/ml for Amlodipine with a correlation coefficient of 0.999, 0.998 and 0.997 respectively. The developed method was validated as per ICH guidelines for linearity, accuracy, precision, robustness, specificity, limit of detection, limit of quantitation.
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