Role of the enzymes derived from isolated bacteria Pseudomonas aeruginosa
The study is included the effects of proteases enzymes producing by Pseudomonas aeruginosa, which isolated from keratitis and wounds. two hundred samples were collected, 150 samples of them were keratitis, and fifty samples were wounds. Seventeen isolates (8.2 %) were obtained and diagnosed as P. aeruginosa.13 isolates (8.5%) from keratitis and 4 isolates (8%) from wound swabs. All bacterial isolates were diagnosed by biochemical tests. The production of elastase was tested by using elastin agar. Thirteen isolates (76.5%) had the ability to producing elastase (10 isolates (76.5%) from keratitis and 3 isolates (76%) from wounds). The isolates P7 and P11from keratitis and P2 from wounds had the larger hydrolysis zone (10, 10, 8 millimetres) respectively. The production of alkaline protease was tested by using solid cultures (skim milk agar), all of them (keratitis and wound swabs) had the ability to produce of the enzyme, the isolates P12 isolated from keratitis had the larger hydrolysis zone (22 millimetres). Also, the production of the enzyme was tested in liquid cultures (casein broth), all isolates from keratitis had the ability to the production of the enzyme, the isolates P12 had the specific activity 97.469 U / mg, while 50% of the wounds isolates had the ability to produce alkaline protease.
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