Main Article Content

Abstract

This research objective is for the development of a specific and simple method to trace Semaglutide presence in active pharmaceutical ingredient and pharmaceutical dosages. As part of a study on Semaglutide drug, solvents of HPLC grade waters HPLC instrument (Empower software) with PDA detector, ultrasonicator (Make: Labman) and pH meter (Make: Adwa) are used. The Method was optimized with mobile phase with a composition of buffer and solvent were of 60:40%v/v, flow maintained was 1.0ml/min, the injection volume of 10µl, run time was 5min. All separations were performed with PDA detector and column used was Discovery C18 150 x 4.6mm, 5m. Results for the developed method are accurate and specific. The detection wavelength was 292 nm, the retention time for Semaglutide was 2.689min, linearity resulted with r2= 0.9998, % RSD for precision was 1.0; %mean recovery for accuracy was in the range of 99.73 to 100.29.  This study report is for industrial application for determining Semaglutide presence in pharmaceutical ingredient and dosages.

Keywords

Semaglutide RP-HPLC Validation Method Development Detection

Article Details

How to Cite
Arun Kumar Kuna, Ganapaty S, & Radha GV. (2019). A novel RP- HPLC method development and forced degradation studies for semaglutide in active pharmaceutical ingredients and pharmaceutical dosage form. International Journal of Research in Pharmaceutical Sciences, 10(2), 865-873. https://doi.org/10.26452/ijrps.v10i2.263