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The purpose of this research is to study the dominant chemical compounds of chicken bile extract. Chicken bile, which is usually disposed of as useless waste, is made a choice. The study began with material collection from native chicken farmers, Kluwut Village, Bulakambah sub-district, Brebes Regency, Central Java. The determination was carried out at Bandungense Herbarium School of Biological Science and Technology-ITB.  The choice of animals were native chickens. They belonged to the family Phylum: Chordata, Class: Aves, Nation: Galliformes, Tribe: Phasianidae, Surname: Gallus, Type: Gallus gallus Linn, The common name: native chicken (Indonesia), and domestic fowl in English. The bile portion was cut into small pieces and dried using a freeze dryer. The reflux method was then extracted using solvents with a different polarity, which are n-hexane, ethyl acetate, and ethanol. It gave out n-hexane extract, ethyl acetate extract, and ethanol extract. The extracts were evaporated using a rotary evaporator. The percentage of the obtained yield was n-hexane extract 7.63%, ethyl acetate extract 8.61%, and ethanol extract 34.91%. Selected ethyl acetate extract was fractionated by Vacuum Liquid Chromatography (VLC I)  and was monitored by a thin layer of Chromatography (TLC). Then fraction 5-6 was continued to sub fractionation by Classical Column Chromatography (CCC). Nuclear Magnetic Resonance (NMR) investigated isolate X and found that isolate X appeared to be cholesterol. From the second fractionation (VLC II), fraction 2-7 proceeded to the VLC III, and then subfraction 7 was evaluated using GC-MS. The dominant chemical compounds of subfraction 7 were oleic acid 38.72%, n-hexadecanoic acid (35.6%), octadecanoic acid (17.94%), palmitoleic acid (1.53%).


chicken bile chemical compound dominant

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How to Cite
Tuty S, Fidrianny I, & Sukrasno. (2020). Dominant chemical compound of chicken bile extract. International Journal of Research in Pharmaceutical Sciences, 11(2), 2632-2637.

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