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Extended Spectrum B-Lactamase producing bacteria has become a worldwide problem and made the treatment very much complicated due to their resistance ability against the wide number of antimicrobial drug. This study was undertaken to measure the emergence of ESBL producing bacteria in urine collected from the patient Chhattisgarh Institute of Medical Science, Bilaspur, C.G. A total of 141 Eterobactericeae groups isolates and identified. A Total of 81 ESBL producing uropathogens were identified. The most prevalence of ESBL producing bacteria was E. coli 59(41.84%) followed by Klebsiella species 12(8.51%), and Enterobacter species were 06(4.26%). ESBL producers are confirmed by the Phenotyping confirmatory disc diffusion test. Antimicrobial susceptibility testing by Kirby-Bauer method showed most resistance were ampicillin (88.9%), Ciprofloxacin (69.1%), amoxycillin clavulanic acid (76.5%) resistance to ESBL producers and least resistant to imipenem 15(18.5%). We are confident that this research will be helpful in human beings and further medical microbiological study.
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