Cytotoxic effects of Ceiba pentandra L. mediated silver nanoparticles on HCT-116 colon cancer cell lines through ROS generation and cell membrane damage

  • Masese Osoro Brian PG and Research Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India
  • Selvi S PG and Research Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India

Abstract

In this study, we assessed the biological effect of Ceiba pentandra bark silver nanoparticles (CP-AgNPs) on HCT-116 cancer cells with an emphasis on cell cytotoxicity, quantification of ROS and determination of mitochondria membrane potential.  The synthesized Ceiba pentandra bark silver nanoparticles were characterized by UV-vis spectrometer, High-resolution transmission electron microscope (HR-TEM), X-ray diffraction (XRD) and Selected area electron diffraction (SAED). The synthesized silver nanoparticle cytotoxic and apoptotic effects were studied on colorectal cancer cells (HCT-116). The nanoparticles exhibited an inhibitory concentration (IC50) at (60μg/mL). CP-AgNPs significantly inhibited cell viability and changed the morphology of HCT-116 colon cancer cells. Moreover, CP-AgNPs increased the level of reactive oxygen species, induced cell apoptosis in HCT-116 cell lines through the interference of the mitochondrial membrane potential. These results indicated that Ceiba pentandra bark silver nanoparticles (CP-AgNPs) might act as prospective anticancer agents in colon cancer cells.

Keywords: Anticancer, C.pentandra, Colon cancer, Silver nanoparticles

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Published
2019-10-16
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How to Cite
Masese Osoro Brian, & Selvi S. (2019). Cytotoxic effects of Ceiba pentandra L. mediated silver nanoparticles on HCT-116 colon cancer cell lines through ROS generation and cell membrane damage. International Journal of Research in Pharmaceutical Sciences, 10(4), 3236-3243. https://doi.org/10.26452/ijrps.v10i4.1627
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