Enantiomeric separation of Midodrine hydrochloride in bulk and pharma-ceutical dosage form by chiral HPLC

  • Jenifer Ashwini S Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India
  • Narenderan ST Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India
  • Meyyanathan SN Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India
  • Babu B Department of Pharmaceutical Analysis, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India
  • Gowramma B Department of Pharmaceutical Chemistry, JSS College of Pharmacy, Udhagamandalam, JSS Academy of Higher Education & Research, Tamil Nadu, India

Abstract

A simple, sensitive chiral liquid chromatographic method was developed for the separation and quantification of enantiomers Midodrine. A chiral PAK IG-3 (150 x 4.6 mm) 3µm column was used for the separation of the enantiomers. The mobile phase consists of 10 mM ammonium bicarbonate in water and acetonitrile in the ratio of 95:5, v/v with a flow rate of 0.7 ml/min. The detection was done at 290 nm with column temperature maintained at 40°C. The method linear ranged between 10 – 110 μg/ml and 5 – 100 μg/ml for (+) and (-) Midodrine enantiomers.  The recovery of the method was found to be in the range of 99.1 to 101.2 %. The detection limit for the (+) and (-) enantiomers was found to be 4 μg/ml and 1 μg/ml, respectively. A simple validated chiral HPLC method with reverse elusion is described for the separation and quantification of the enantiomers of Midodrine in bulk and formulation.

Keywords: Midodrine, Enantiomers, HPLC, Validation, ICH

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Published
2019-07-12
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How to Cite
Jenifer Ashwini S, Narenderan ST, Meyyanathan SN, Babu B, & Gowramma B. (2019). Enantiomeric separation of Midodrine hydrochloride in bulk and pharma-ceutical dosage form by chiral HPLC . International Journal of Research in Pharmaceutical Sciences, 10(3), 1608-1611. https://doi.org/10.26452/ijrps.v10i3.1320
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Original Articles
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