Preliminary phytochemical screening, evaluation and comparison of anti-cancer activity of Ficus gibbosa blume by MTT assay method

  • Gini E.J Chemists College of Pharmaceutical Sciences and Research, Varikoli, Kerala, India
  • Sivakumar T Annamalai University, Chidambaram, Tamil Nadu, India
  • Kuppuswamy S Nirmala College of Pharmacy, Muvattupuzha, Kerala, India

Abstract

The present study involves the preliminary phytochemical screening and evaluation of anticancer activity of the whole plant Ficus gibbosa blume .Extracion was carried out using the solvents petroleum ether, ethyl acetate, chloroform, ethanol and water. Among this, aqueous and ethanolic extract contains more active constituents, hence selected for the study. MTT assay method (invitro) was used for the evaluation of anti-cancer activity. Hacat and Mcf-7 cell lines were used for the evaluation. Preliminary phytochemical screening showed the presence of alkaloids, glycosides, saponins, flavonoids, tannins, steroids, carbohydrates and proteins. Aqueous extract shows better activity against both Hacat and Mcf-7 cell lines and as the concentration increases there is an increase in the cell growth inhibition. For aqueous extract the IC50 value was found to be 46.54µg/ml for Hacat and 92.4µg/ml for Mcf-7 cell lines. The present study concludes that both aqueous and ethanolic extract of entire plant of Ficus gibbosa blume possess anticancer activity against Hacat and Mcf-7 cell lines.

Keywords: Cytotoxic, Ficus gibbosa blume, linear regression analysis, MTT assay, Soxhlet extraction

Downloads

Download data is not yet available.
Published
2016-07-20
Citations
How to Cite
Gini E.J, Sivakumar T, & Kuppuswamy S. (2016). Preliminary phytochemical screening, evaluation and comparison of anti-cancer activity of Ficus gibbosa blume by MTT assay method . International Journal of Research in Pharmaceutical Sciences, 7(3), 190-194. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/1265
Section
Original Articles
Article Level Metrix
Abstract Views: 3
PDF Downloaded: 2