Apigenin reduces cell viability of Human epidermoid cancer cells (Hep-2)

  • Shanmugam Manoharan Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India
  • Tharmaraj Rejitharaji Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India
  • Murugaraj Manoj Prabhakar Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India
  • Sekar Karthikeyan Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India
  • Villianur Ibrahim Hairul Islam Pondicherry Centre for Biological Sciences, Boomyinpet, Puducherry-5, India

Abstract

The aim of the present study is to explore the cytotoxic potential of apigenin in Hep-2 cells. The mechanistic pathway for its cytotoxic potential was analyzed using MTT assay (cytotoxicity), DNA fragmentation (agarose gel electrophoresis), Nuclear damage (DAPI Staining), ROS generation ability (DCFH-DA method), apoptosis inducing potential (dual staining) and expression of apoptosis and angiogenic proteins (Western blotting). The overall findings of the present study suggest that apigenin might have reduced the cell viability of Hep-2 cells through its apoptotic and anti-angiogenic potential.

Keywords: Apigenin, apoptosis, cell viability, Hep-2

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Published
2014-07-25
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How to Cite
Shanmugam Manoharan, Tharmaraj Rejitharaji, Murugaraj Manoj Prabhakar, Sekar Karthikeyan, & Villianur Ibrahim Hairul Islam. (2014). Apigenin reduces cell viability of Human epidermoid cancer cells (Hep-2) . International Journal of Research in Pharmaceutical Sciences, 5(3), 152-158. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/1190
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