Antibacterial activity of silver nanoparticles synthesized using Amaranthus viridis twig extract

  • Rama Koyyati Department of Biochemistry, Osmania University, Hyderabad - 500 007, India
  • Veera babu Nagati Department of Biochemistry, Osmania University, Hyderabad - 500 007, India
  • Raju Nalvothula Department of Biochemistry, Osmania University, Hyderabad - 500 007, India
  • Ramchander Merugu Department of Biochemistry, Osmania University, Hyderabad - 500 007, India
  • Karunakar Rao Kudle Department of Biochemistry, Osmania University, Hyderabad - 500 007, India
  • Paul Marx Department of Biochemistry, Osmania University, Hyderabad - 500 007, India
  • Pratap Rudra Manthur Padigya Department of Biochemistry, Osmania University, Hyderabad - 500 007, India

Abstract

Development of nano biotechnology is generating interest of researchers toward ecofriendly, cost effective and biological synthesis of nanoparticles. In this study, synthesis of stable silver nanoparticles was done using Amaranthus viridis twig extract. These rapid biological synthesized silver nanoparticles were characterized with the help of UV–Vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy-Energy dispersive spectroscopy (SEM-EDX), Transmission Electron Microscopy (TEM) and X-Ray diffraction spectroscopy (XRD). Stability of reduced silver nanoparticles was analyzed using UV–Vis spectra shown that the absorption peak, occurring due to Surface Plasmon Resonance (SPR), exists at 423.5nm and their antibacterial activity was screened against both gram-negative and gram-positive bacteria. It was observed that Amaranthus viridis twig extract reduced silver ions into silver nanoparticles within 10 min of time. Thus, this method is rapid and ecofriendly. The TEM and SEM analysis inferred that the rapid biologically synthesized silver nanoparticles are spherical in shape and have an average size of 5-20nm. The antibacterial activity of silver nanoparticles against both gram positive and gram negative bacteria was performed in Luria–Bertani (LB) medium on solid agar plates and in liquid systems supplemented with different concentrations of nano-sized silver particles. These particles were shown to be an effective bactericide. Test for protein and nucleic acids leakage were performed to study the biocidal action of this nano sized particles. The results confirmed that the treated bacterial cells were damaged, showing leakage of proteins and nucleic acids into LB media. These nanoparticles, which can be prepared in a simple, rapid and cost-effective manner, are suitable for the formulation of new types of bactericidal materials.

Keywords: Amaranthus viridis twig extract, UV–Vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), Energy dispersive spectroscopy (EDX), Transmission electron microscopy (TEM), X-Ray diffraction spectroscopy (XRD), antibacterial activity, biocidal action

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Published
2014-01-25
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How to Cite
Rama Koyyati, Veera babu Nagati, Raju Nalvothula, Ramchander Merugu, Karunakar Rao Kudle, Paul Marx, & Pratap Rudra Manthur Padigya. (2014). Antibacterial activity of silver nanoparticles synthesized using Amaranthus viridis twig extract . International Journal of Research in Pharmaceutical Sciences, 5(1), 32-39. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/1156
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