Estimation of Valethamate bromide in pharmaceutical dosage forms by HPTLC

  • Venugopal V Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Harshita M Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Sowjanya G Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Nikitha B Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Vikram B Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Sunil L Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Kanaka Prasad M Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India
  • Subhash G Department of pharmaceutical analysis, SLC’s college of pharmacy, Hyderabad, India

Abstract

A simple, rapid, sensitive and highly precise High Performance Thin Layer Chromatographic Method has been developed for the estimation of Valethamate bromide in bulk and pharmaceutical dosage forms. HPTLC was performed on CAMAGLINOMAT IV, TLC scanner version 3.20, using n-butanol and glacial acetic acid (9:1) (v/v) gas mobile phase. The Chromatogram was developed in CAMAG twin trough glass chamber containing mobile phase. The TLC plates were scanned at 299nm in schimadzu dual wavelength scanner, and Rf value of valethamate bromide was found to be 0.37. The LOD and LOQ values were found to be 6µg/ml and 18µg/ml respectively. The linearity of Valethamate bromide was found to be 20-100µg/ml and gives correlation coefficient of 0.9985.

Keywords: Valethamate bromide, Methanol, Glacial acetic acid, n-butanol, HPTLC, Validation

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Published
2012-07-25
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How to Cite
Venugopal V, Harshita M, Sowjanya G, Nikitha B, Vikram B, Sunil L, Kanaka Prasad M, & Subhash G. (2012). Estimation of Valethamate bromide in pharmaceutical dosage forms by HPTLC. International Journal of Research in Pharmaceutical Sciences, 3(3), 373-375. Retrieved from https://pharmascope.org/index.php/ijrps/article/view/1049
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Original Articles
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