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A simple, precise, accurate RP-HPLC method has been developed for the simultaneous estimation of cefixime and ofloxacin in combined tablet dosage form. The chromatographic separation was achieved using Phenomenex Luna C18 (250 X 4.60 mm, 5 µm) analytical column and the mobile phase consisting of methanol and 25 mM phosphate buffer 40: 60% (v/v) pH adjusted to 5.5 using ortho phosphoric acid at a flow rate of 1.2 mL/min. The UV detection was carried out at 290 nm using photodiode array detector. The retention time of cefixime and ofloxacin was found to be 2.5 min and 7.8 min respectively. The proposed method was validated for specificity, linearity, accuracy, precision, ruggedness, robustness, limit of detection and limit of quantitation. Linear calibration curves were obtained in the concentration of 5-25 µg/mL for cefixime and ofloxacin. The mean percentage recovery obtained for cefixime and ofloxacin were respectively between 98.0% and 105.7%. Limit of detection and quantification for cefixime were 13.3 ng/mL and 40.1 ng/mL and for ofloxacin 12.3 ng/mL and 37.1 ng/mL, respectively. The developed method can be used for routine analysis of titled drugs combination in tablet formulation.
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