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Insufficient studies had been carried on the effectiveness of (Phoenix dactylifera) Ajwah date in the healing of burns. The aim of the current study was to inspect the role of Ajwah date, honey and low intensity laser therapy in healing of burn as well as antimicrobial activity. Total Flavonoids contents in methanol extract of Ajwah date with an average value of 5.574 mg QE/gram. Quercetin described as the greatest flavonoids contents within the extract (2.438 mg GAE/gram fw). Moreover, quercetin extracted to examine the antimicrobial activity via TLC and recognized by IR, MS, and 1H and 13C NMR. Quercetin of Ajwah date presented different responses through creating numerous inhibition zones against examined microorganisms. Chemical burn was induced to twenty local rabbits that were divided randomly into four groups of equal number received topical application of quercetin (80 %), lime honey, low intensity laser therapy (LILT) and control group. Wound surface area was measured through tracing method and 3D camera while wound swab was used to detect the microbial load. The results revealed that there were significant differences in wound surface area and microbial load for the treatment groups when compared to the control group after 7, 14, and 21 days as p<0.05, while the quercetin group was superior to honey and low intensity laser therapy respectively. This study supported that quercetin extracted from ajwah; honey and LILT are beneficial methods to accelerate the wound healing process as well as decrease the microbial load.
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