A stability indicating RP-HPLC method for simultaneous estimation of Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz in pharmaceutical dosage forms

Abstract

A stability indicating RP-HPLC method has been developed for quantitative determination of Emtricitabine, Tenofovir Disoproxil Fumarate and Efavirenz in Pharmaceutical dosage forms. Chromatographic separation was achieved through gradient elution. Detection wavelength was monitored at 262 nm. The retention times of the Emtricitibine, Tenofovir disoproxil fumarate and Efavirenz was about 2.6, 5.4, 7.9min respectively. The developed method was validated as per ICH guidelines. This method is found to be simple, fast and economical Hence this validated method can be used in routine quality testing of individual dosage forms and combination dosage forms of Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz.