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In this study, Proteolytic keratinase was observed from Streptomyces sp., isolated from poultry plant soil and purified by ammonium sulfate fractionation method followed by Gel filtration chromatography. The purified enzyme was further characterized partially which covers the influence of pH, temperature, incubation time, nitrogen sources and metal ions on the activity of proteolytic keratinase. From the results, it was observed that, proteolytic keratinase isolated from the Streptomyces sp. enumerated from the poultry plant soil showed maximal activity at pH 8.0 and at temperature 50oC within 55mins. It was also found that, at the minimum concentration of 2mM BaCl.2H2O, CuSO4.5H2O and MgCl2, it showed highest keratinase activity.
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