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In recent years' nanoparticle have attracted interest because of their wide application in biomedicine. Nanotechnology has acquired interest because of their eco-friendly approach for the synthesis of the silver nanoparticle. This work demonstrates the efficacy of biologically synthesised Ag nanoparticle by means of Cynodon dactylon. Phytochemical analysis of C. dactylon suggests the presence of active constituents in polar solvents. Agnps were optimized using different parameters, and in general, the effect for temperature was 75°C within 1 hr, in a neutral condition, the concentration was found to be 1mM. Uv- vis analysis of synthesized silver nanoparticle indicates that the absorption peak was observed at 240nm. Absorbance peak at 2917cm-1, 1648cm-1 indicate the presence of capping agent responsible for synthesis. A broad spectrum of antimicrobial activity was reported in 400µl of synthesised silver nanoparticle (23.3 mm) against P. aeuroginosa, followed by Bacillus sp. (22.8 mm) and F. oxysporum (22.1 mm). HPLC analysis indicates the presence of active constituents in the sample. This study reveals that C. dactylon acts as a potential source for nanoparticle synthesis.