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Contamination of food by food handlers contributes to vast food outbreaks due to the transmission of pathogens from the food handlers' hands. Thus, hand hygiene is a great manner to reduce the infections from harmful pathogens. The aims of this study were to assess the hand hygiene practice among food truck vendors based on a self-reported questionnaire and direct observation. A cross-sectional study was conducted among food truck vendors at Klang Valley. The self-reported questionnaire and direct observation were used to identify the hand hygiene practice and the significant difference of outcomes between these two instruments. Cohen kappa reveals a poor agreement between self–reported questionnaire and direct observation on hand hygiene practice among respondents. The mean difference on hand hygiene practices for the self-reported questionnaire and direct observation among the food handlers were 17.77±3.15 and 11.45±3.00 respectively, where a significant difference was observed (p=0.003). The results revealed that over-reporting errors should be considered when analyzing and interpreting the data derived from the self-reported survey. The outcome also indicated that food safety education and enforcement is necessary for promoting food safety practice.
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