Molecular detection for Trichomonas virginals in placenta tissue from women with BOD

Abstract

Trichomonas viginalis is a unicellular parasite, occur in one stage (Trophozoite) and transmitted to human by sexual activity. Trichomoniasis is common in sexually active women of all ages. Disease symptoms range from asymptomatic to severe inflammation. Also, studies indicated that trichomoniasis implicated in causing adverse pregnancy outcomes. In this study, we try to investigate this effect in women with BOD history by the molecular method. Among the selected pregnant women, two hundred placental tissues were collected with a history of "BOD" that is sent to outpatient gynaecology departments, units of the Al Alamad Al Kadhimain Medical City Crisis Unit and the Fatima Al-Zahraa University Hospital from December 2015 to May 2016 Baghdad, Iraq. The tissues were homogeneous, and the DNA was extracted. Two genes were used for the detection of Trichomonas by conventional PCR. The mean age for two hundred women enrolled in this study were 27 ± 6.6. One hundred fourth of them had abnormal pregnancy outcomes (62%). The main abnormal outcomes were abortion (36%), stillbirth (18%) and congenital abnormality (8%). By the conventional PCR, Trichomonas viginalis was detected in 24 /200 (12%) of the total placental tissues. 75 % were in age 20-29. Nine of infected women with Trichomonas vaginalis had Abnormal pregnancy, the majority of abnormal outcomes were abortion (29.17%), stillbirth (8.33%) and no congenital abnormalities were detected. 54.17% had 3-4 previous pregnancies. All the results were significantly important. Trichomonas vaginalis can reach the placental tissue, and there is a clear association with a woman who had a BOD.