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Piper Linn. (Black pepper) belongs to the family Piperaceae and an economically and medicinally important spice and is a native of Southern India. The gall tissues have shown various structural and physiological changes in the host tissues. The normal and gall tissue showed differential in terms of the metabolites and enzymes. The Diptera comprises a large group of insects including the common flies, mosquitoes, gnats and midges are by the position of only the pair of wings. Some of them are , others feed on nectar or plant sap and decaying animal and vegetable matter and few others are blood sucking. When feeding on plant tissues these insects and mites inject or secrete a chemical substance into the plant that causes the plant to grow abnormally and produce a gall. Stimulus for gall formation is usually provided by the feeding stage of the insect. in the gall as until the completion and maturity of their life stages and emerges from the exit holes. present investigation, The intensity of starch, cellulose, carbohydrates, proteins, lipids, lignin, and the viz. acid , and oxidase was observed in diseased leaf of Piper and estimated and the results have been discussed in the light of , induced by Diptera.
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