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Amylose based chiral HPLC method for enantioselective separation of ondansetron was developed, optimized and validated on Chiral Pak AS-3R analytical column (150 mm × 4.6 mm i.d., 3 μm). Mobile phase comprising of Acetonitrile: 0.2% Triethylamine: Methanol (15.49:30:54.51) and at a flow rate of 1.33mL/min. The separation was detected at a wavelength of 254 nm and run time was achieved within 6.0 min. Applied Central composite design (CCD) to validate the impact of three independent variables: 0.2% triethylamine (TEA), Acetonitrile (ACN) and flow rate (FL) keeping the methanol concentration constant and the responses selected were capacity factor (k1), resolution (Rs2,1), and retention time (tR2). Using this approach, a mathematical model was elucidated and response surface plots were acquired for the separation. CCD results were integrated into a multi-criteria decision making approach in order to prevail a set of optimal experimental conditions directing to the most desirable compromise between resolution and analysis time. This method can be applied for the routine analysis.


Chiral separation Central composite Derringer’s desirability Multicriteria decision Ondansetron

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How to Cite
Ganna Anitha, & Pandey VP. (2018). Enantioseparation and purity determination of Ondansetron by amylose based Chiral HPLC method: A Chemometric approach. International Journal of Research in Pharmaceutical Sciences, 9(3). Retrieved from