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A novel, simple, accurate reverse phase ultra-performance liquid chromatographic method for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine in pharmaceutical dosage form was developed and validated. This method was performed on Thermo scientific-Hypersil gold C18, 1.9µm (50mm X 2.1mm) column with Mobile Phase 60 volume of buffer and 40 volume of Acetonitrile. Buffer Solution was mixing 1ml of O-Phosphoric acid in 1000ml of water. The flow rate was 0.3 ml/min and detection were carried out using a PDA detector at 232 nm. The retention times were 2.6, 3.0, & 3.7 min for Chlorthalidone, Telmisartan and, Amlodipine respectively. The method was linear over the concentration range of 8-12μg/ml for Chlorthalidone and 24-36µg/ml for Telmisartan and 3-7µg/ml for Amlodipine with a correlation coefficient of 0.999, 0.998 and 0.997 respectively. The developed method was validated as per ICH guidelines for linearity, accuracy, precision, robustness, specificity, limit of detection, limit of quantitation.


Telmisartan Chlorthalidone Amlodipine besylate RP-UPLC Simultaneous Estima-tion

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Sivakamasundari G, & Kannappan N. (2018). Method development and validation for simultaneous estimation of Chlorthalidone, Telmisartan and Amlodipine by RP-UPLC in pharmaceuti-cal dosage form. International Journal of Research in Pharmaceutical Sciences, 9(3). Retrieved from