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The highest pectinase producing isolate A3 out of forty isolates of Pantoea sp. bacteria was subjected to physical effects by using UV light to change in their ability for producing of pectinase, the results appeared increasing their activity and production in some of the mutants more than wild type bacteria, the increase was ranged between 14 to 20% in specific activity between bacterial mutants compared with wild type bacteria. Also, the two highest enzyme-producing isolates were tested for their susceptibility against a group of antibiotics (Rifampin 50mcg, Imipenem 10mcg, Vancomycin 30mcg, Amoxicillin 25mcg, Chloramphenicol 30mcg, Trimethoprim 5mcg, Nitrofurantoin 100mcg, Nalidixic acid 30mcg), results appeared different susceptibilities ranged between resist for amoxicillin,and Nalidixic acid, and sensitive for other antibiotics. The susceptibilities of studied bacteria to some antibiotics were different between resist and sensitive, which considered an indicator of dangerous to human life. The study was aimed to increase the ability of these isolate in producing this enzyme which plays an essential role in different industries in food, cosmetics manufacturing and scientific research, besides, their ability in resisting some of the common antibiotics to confirm the transferring of genetic materials between bacteria that isolated from soil samples, which is considered crucial in the microbial genetics. The study appeared there were apparent effects on the genes that responsible for enzyme production either for increase or decrease their activity and creation of an enzyme, which belong to using physical effects to improvement these feature in bacteria.
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