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To determine the effect of a natural extract of of 2.5% concentration on the of root canal when used as an irrigating solution. This was an in comparative study where and twenty intact single-rooted teeth were selected and with a diamond bur to get an apical-coronal length of 10 mm and were randomly separated into three groups as per the used; Group 1 (control): Irrigation with Normal saline, Group 2: Irrigation with Smear Clear solution, Group 3: Irrigation with 2.5% extract solution. were prepared using Pro Taper Universal Rotary Files with intermittent irrigation with the respective irrigating solution. The teeth were then embedded in acrylic resin and subjected to Vicker's Hardness test, and the data obtained were analyzed using one way ANOVA. When Group 2(51.80±9.80 VHN and 52.66±5.28 VHN) was compared with Group 3(44.98±4.34 VHN and VHN) the values showed a statistically significant difference (p< 0.05).When Group 3 (44.98±4.34 VHN and VHN) was compared with Group 1(65.37±8.80 VHN and 67.74±9.56 VHN), the values did show a statistically significant difference (p> 0.05). Within the limitations of this study, teeth irrigated with normal saline showed the least reduction in followed by irrigation with leaf extract solution and Smear Clear.
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