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Abstract

A novel RP-HPLC validated method for determination of Exemestane is developed. The chromatographic separation was done on Phenomenex Luna reversed phase C18 (150 mm x 4.6 mm, 5 μm) column in isocratic mode, using Acetonitrile: HPLC grade water (50:50, v/v) as mobile phase at 254 nm wavelength. Exemestane chromatographic peak is eluted with retention time 4.656 min. The linearity range was 5-30 μg/ml with correlation coefficient 0.999. The method was validated as per ICH Guidelines. The quantification and detection limit for estimation of Exemestane was found to be 0.365 and 1.926 μg/ml respectively. Recovery of Exemestane was found in the range of 98.0-102.0%.

Keywords

RP-HPLC Exemestane Validation Estimation

Article Details

How to Cite
Siva Sai Kiran B, & Raja S. (2018). Development of validated method for the determination of exemestane by using RP-HPLC. International Journal of Research in Pharmaceutical Sciences, 9(1), 185-188. Retrieved from https://pharmascope.org/ijrps/article/view/212