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In the present study, a Streptomyces sp. was isolated from sediment soil samples of the Kali river estuary, Karwar, Karnataka, India. The isolated Actinomycetes strains were screened against pathogenic microorganisms and for the production of hydrolytic enzymes. Later, the strongest strain Streptomyces sp.KAS-1 was taken for further analysis based on primary characterizations. The morphological, physiological and biochemical characterizations of Streptomyces sp.KAS-1 were carried out, followed by molecular characterization through 16S rRNA gene sequencing. The 16S ribosomal RNA gene sequencing and analysis of the phylogenetic tree showed a 98.97%sequence similarity with Streptomyces thermocarboxydus strain SDT64(KJ018992). Finally, the intracellular methanol extract was characterized through UV-Visible and FTIR spectrophotometer. The Streptomyces sp.is a gram-positive, aerobic, non-motile, spore-forming bacterium and it was designated as Streptomyces sp.KAS-1 and was found to possess a strong antimicrobial activity against pathogenic microorganisms. The characterizations revealed the Streptomyces sp.KAS-1was moderately thermophilic, neutrophile organism having the capacity to degrade a broad range of carbon sources. The UV-Visiblespectrum ofmethanol extract of Streptomyces sp.KAS-1revealed the presence of Muconomycin-Band the presence of different functional groups such as aldehydes, amines, and alkyl halides were indicated by the FTIR spectrum.
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