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The G.glabra is otherwise called liquorice is a medicinal plant is used for various diseases like cold, cough, hypokalemia and muscle weakness, etc. The liquorice family belongs to the Fabaceae family of the G.glabra. Hence this study tells about the anti-inflammatory and antioxidants. The quantitative study of phytochemical analysis, antioxidant and Anti-inflammatory, cytotoxicity assay using a response from the root extract of G.glabra The results are showed above preliminary activity phytochemicals were present Alkaloids, Flavonoids, Coumarin, Saponins, Terpenoids, Steroids, Cardiac Glycosides. The antioxidant activity of aqueous extract of G. glabra were evaluated with the 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide radical (NO), superoxide radical (SO), hydrogen peroxide radical (H2O2), and hydroxyl radical (HO) scavenging activity. This study shows about the activity of Glycyrrhiza glabra herbal medicinal plant aqueous extract using antioxidant assays, Anti-inflammatory assays. The in vitro MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Cytotoxicity assay were studied GG(G.glabra) extract using the macrophages Raw 246.7 Cell line. The cell line assay were studied in two different activity. One is anti-inflammation studies, and another is cytotoxicty of GG aqueous extract. The anti-inflammation IC50 value is 143.65, and GG extract dry sample were against the IC50 value is 326.27. From above, the results were a potential activity of GG extract R-value 0f 0.991.
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