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The biggest and most multifaceted class of organic compounds includes heterocyclic compounds. Currently, several heterocyclic compounds are identified, and persistently gratefulness to tremendous synthetic work and synthetic usefulness, the number is increasing exponentially. In most fields of science, including medicinal, pharmaceutical, and agro-chemistry, heterocyclic compounds have a function, and biochemistry is also another area. In this research article, the green approach is administered for achieving the nitrogen, oxygen and sulphur centered five-membered heterocyclic derivatives. By taking the whole thing into account of hetero-chemistry, the moderately effective analog for gram-positive and gram-negative strains was shown for the five-membered heterocyclic compound series of N-substituted iminothiazodine-4-one and N-(benzylideneamino)thiazol-4(5H). The compound 6b showed very much active potency in accordance with the type standard drug the 6c compound against gram-positive Bacillus subtilis bacteria compared to the standard drugs and 6b indicated very active potency against the gram-negative Escherichia coli bacterial strain. The 5a and 6a compounds showed very strong activity against the fungal strain of Candida albicans and 6b or 6c were more active and highly potent than the standard drugs against Aspergillus niger species. In the view of this research, drive states that all the synthesized compounds might be used for the development for further heterocyclic entities.
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