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“Mesenchymal Stem cells (MSCs)” are distinct type of cells naturally present in the body that has the ability to separate into any cell type like bone, cartilage, muscle, nerve, tooth etc. There are numerous studies available to efficiently isolate culture, characterize and expand the bone marrow mesenchymal stem cells. Similar such method was utilized to isolate the MScs from femoral and tibial bone of female wistar rats and the standardized method was used to culture and expand MSCs. The cultured cells were determined to be MSCs with their hallmark properties of spindle shape morphometry, cellular plasticity & cell adherence property. The cells after expansion were subjected to flow cytometry analysis for characterization. The BMSCs showed positive expression for CD90 & negative expression for CD34. In the present investigation, we obtained the BMSCs that would have potential clinical significance as it was performed in a GMP environment with all necessary requirements that could enable its therapeutic value.
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