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In this study, an effort was performed to produce the data on, physicochemical parameters, preliminary phytochemical screening, histochemical studies, and HPTLC data required for the standardization of Balamula Churna concerning root powder of Sida acuta. The physicochemical parameters such as water-soluble extractive, alcohol soluble extractive, and loss on drying at 105 °C, total ash, and insoluble acid ash were determined according to standard methods. HPTLC studies were carried, and Rf values were documented. Physicochemical parameter values were recorded as pH (6.73 LOD (114.2gms) water-soluble extractive value (4.48%), alcohol soluble extractive value 6.88%) total Ash (11.00%) and Acid Insoluble Ash (2.72%). Preliminary phytochemical screening reveals the presence of glycoside, alkaloid, carbohydrate, phenol, flavonoid, and saponin. HPTLC screening showed the occurrence of significant phytoconstituents with Rf values 0.07, 0.21, 0.54, and 0.86. The obtained results from this study can be effective in the evaluation of quality, detection of adulteration and substitution, and highlighting the importance of standardization
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