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Spectrophotometric determination of irreversible proton pump inhibitor Rabeprazole sodium has been developed, the procedure is based on the formation of 2:1 chelate of drug and metal in ethanol media to form an orange colour chelate having an absorption maximum at 455nm. The regression analysis of Beer’s law showed a good correlation in the concentration range of 10 - 60µg/ml of pure Rabeprazole with Iron (Fe+3). The limit of detection was 1.98µg/ml and limit of quantification was 6.59µg/ml. The optimum assay conditions were investigated and the recovery of the drug from their dosage forms was found to be 99.8 – 100.3%. Good values of precision were obtained, intraday R.S.D was 0.1567 and interday R.S.D was 0.1792.


Rabeprazole sodium Spectrophotometer IronIII (Fe 3) metal chelate validation

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How to Cite
D. Madhuri, K.B. Chandrasekhar, M. Ramakotaiah, G. Somasekhar, K. Harinadhababa, & K. Ravi Kumar. (2010). Validation of Spectrophotometric determination of Rabeprazole using Ferric Chloride (FeCl3). International Journal of Research in Pharmaceutical Sciences, 1(2). Retrieved from