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The aim of the research is to access the Anti-fungal activity of Neem and Aloe Vera composition mediated zirconium oxide nano particles. The procedure includes a collection of Azadirachta indica (neem) and Aloe Vera plant leaves and preparation of plant extract, Synthesis of zirconium oxide nanoparticles using a herbal formulation and Anti-fungal activity determination of synthesised zirconium oxide nanoparticles. The objective is to evaluate the activity done by neem and Aloe Vera composition mediated zirconium oxide nano particles against fungal infection. As a result, the addition of nanoparticles to prepared plant extracts showed mild or no colour change. Thus zirconium oxide nanoparticles are colourless in nature. But turbidity in solution appears after the addition of nanoparticles. Graphical representation of the synthesis of nanoparticles showed a peak at 350 nanometers(nm) in both initial and final readings, but the rate of absorbance showed a mild increase in final reading which confirms the synthesis of the nanoparticle. The anti-fungal activity was checked against 3 pathogenic fungi Aspergillus fumigatus, Aspergillus niger, Candida albicans and it showed mild ring of a zone of inhibition around the well. Zone of inhibition increases with an increase in the quantity of nanoparticles in the well. Zone of inhibition proves the anti-fungal activity. To conclude, Azadirachta indica (neem) and Aloe Vera composition mediated zirconium oxide nanoparticles show anti-fungal activity.
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