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A new, simple, fast, precise and efficient reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the validation of herbal drugs .Gymnena slyvestrae is a medicinal plants. The objective of the present Gymnema sylvestrae investigation was to isolate and characterize the Gymnemic acid, from marker compound of leaf powder with different solvent systems like petroleum ether, benzene, and methanol. The leaf powder were extracted under continuous hot extraction in Soxhlet apparatus with 90% methanol gave the maximum. Yield of gymnemic acid (42%) from the ecotype collected from Tirupathi .gymnemic acid was purified by preparative chromatographic methods i.e., RP-HPLC and HPLC. The analysis by HPLC showed30% purity of Gymnemic acid
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