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The compounds which is isolated from Leptadenia reticulata and Lawsonia inermis is sample 1 (6-amino-3-hydroxy-4-(4'-methylphenyl)-2Hchromen-2-on) and -sample 2 (3, 7, 4’, 5'-Tetrahydroxy-6- methoxyflavone). These compounds were undergone acute toxicity studies for dose fixation. In acute toxicity studies feminine (8-week old) young Swiss albino mice 20-25 g weight is used. The animals are grouped and in each group 3 animals are used. Generally acute toxicity study is the preliminary study for conducting animal experiments, it is mainly done for dose fixation. The dose fixation is mainly categorized for safer doses. The minor changes and variations in sexes will play a role in animal studies, but the feminine will be typically adoptable for this animal experiments because it is more sensitive than males. After the toxicity study is done the LD 50 value is calculated by having a note on mortality determination. The LD50 value which is calculated as 200mg/kg and gross behavioral studies like respiratory parameters, salivary secretions, sense of touch and sound, writhing reflex, tremor, convulsions, hind limb paralysis, GIT motility and induction of diarrhea were explained. The acute toxicity were done according to OECD Guidelines 423.
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