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Abstract

A new, simple, and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative determination of Acyclovir. Acyclovir was chromatographed on silica gel 60 F254 TLC plate using chloroform: methanol: formic acid (6.5 + 3.5 + 0.1 v/v/v) as mobile phase. Acyclovir was quantified by densitometric analysis at 259 nm. The method was found to give compact spots for the drug (Rf = 0.25±0.01). The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9996 in the concentration range 100–600 ng/spot. The method was validated for precision, recovery, repeatability, and robustness as per the International Conference on Harmonization guidelines. The minimum detectable amount was found to be 100 ng/spot, whereas the limit of quantitation was found to be 300 ng/spot. Statistical analysis of the data showed that the method is precise, accurate, reproducible, and selective for the routine quality control of Acyclovir.

Keywords

Acyclovir Estimation HPTLC

Article Details

How to Cite
Dhobale Shankar Maruti, & Subir Kumar Banerjee. (2013). Development and validation of HPTLC method for estimation of acyclovir in formulations . International Journal of Research in Pharmaceutical Sciences, 4(2), 310-315. Retrieved from https://pharmascope.org/ijrps/article/view/1169